The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix.
Identifieur interne : 000400 ( Main/Exploration ); précédent : 000399; suivant : 000401The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix.
Auteurs : Joshua C D. Long [Royaume-Uni] ; Ervin Fodor [Royaume-Uni]Source :
- Journal of virology [ 1098-5514 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Fibroblastes, Microscopie électronique, Mitochondries (), Protéines adaptatrices de la transduction du signal (déficit), Protéines adaptatrices de la transduction du signal (métabolisme), Protéines virales (analyse), Protéines virales (génétique), Signaux de triage des protéines, Souris, Souris knockout, Sous-type H1N1 du virus de la grippe A (enzymologie), Transport de protéines.
- MESH :
- analyse : Protéines virales.
- déficit : Protéines adaptatrices de la transduction du signal.
- enzymologie : Sous-type H1N1 du virus de la grippe A.
- génétique : Protéines virales.
- métabolisme : Protéines adaptatrices de la transduction du signal.
- Animaux, Fibroblastes, Microscopie électronique, Mitochondries, Signaux de triage des protéines, Souris, Souris knockout, Transport de protéines.
English descriptors
- KwdEn :
- Adaptor Proteins, Signal Transducing (deficiency), Adaptor Proteins, Signal Transducing (metabolism), Animals, Fibroblasts, Influenza A Virus, H1N1 Subtype (enzymology), Mice, Mice, Knockout, Microscopy, Electron, Mitochondria (chemistry), Protein Sorting Signals, Protein Transport, Viral Proteins (analysis), Viral Proteins (genetics).
- MESH :
- chemical , analysis : Viral Proteins.
- chemical , deficiency : Adaptor Proteins, Signal Transducing.
- chemical , genetics : Viral Proteins.
- chemical , metabolism : Adaptor Proteins, Signal Transducing.
- chemistry : Mitochondria.
- enzymology : Influenza A Virus, H1N1 Subtype.
- Animals, Fibroblasts, Mice, Mice, Knockout, Microscopy, Electron, Protein Sorting Signals, Protein Transport.
Abstract
The polymerase basic 2 (PB2) subunit of the RNA polymerase complex of seasonal human influenza A viruses has been shown to localize to the mitochondria. Various roles, including the regulation of apoptosis and innate immune responses to viral infection, have been proposed for mitochondrial PB2. In particular, PB2 has been shown to inhibit interferon expression by associating with the mitochondrial antiviral signaling (MAVS) protein, which acts downstream of RIG-I and MDA-5 in the interferon induction pathway. However, in spite of a growing body of literature on the potential roles of mitochondrial PB2, the exact location of PB2 in mitochondria has not been determined. Here, we used enhanced ascorbate peroxidase (APEX)-tagged PB2 proteins and electron microscopy to study the localization of PB2 in mitochondria. We found that PB2 is imported into mitochondria, where it localizes to the mitochondrial matrix. We also demonstrated that MAVS is not required for the import of PB2 into mitochondria by showing that PB2 associates with mitochondria in MAVS knockout mouse embryo fibroblasts. Instead, we found that amino acid residue 9 in the N-terminal mitochondrial targeting sequence is a determinant of the mitochondrial import of PB2, differentiating the localization of PB2 of human from that of avian influenza A virus strains. We also showed that a virus encoding nonmitochondrial PB2 is attenuated in mouse embryonic fibroblasts (MEFs) compared with an isogenic virus encoding mitochondrial PB2, in a MAVS-independent manner, suggesting a role for PB2 within the mitochondrial matrix. This work extends our understanding of the interplay between influenza virus and mitochondria.
DOI: 10.1128/JVI.01384-16
PubMed: 27440905
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000046
- to stream PubMed, to step Curation: 000046
- to stream PubMed, to step Checkpoint: 000041
- to stream Ncbi, to step Merge: 000C96
- to stream Ncbi, to step Curation: 000C96
- to stream Ncbi, to step Checkpoint: 000C96
- to stream Main, to step Merge: 000400
- to stream Main, to step Curation: 000400
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix.</title><author><name sortKey="Long, Joshua C D" sort="Long, Joshua C D" uniqKey="Long J" first="Joshua C D" last="Long">Joshua C D. Long</name><affiliation wicri:level="4"><nlm:affiliation>Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Sir William Dunn School of Pathology, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author><author><name sortKey="Fodor, Ervin" sort="Fodor, Ervin" uniqKey="Fodor E" first="Ervin" last="Fodor">Ervin Fodor</name><affiliation wicri:level="4"><nlm:affiliation>Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom ervin.fodor@path.ox.ac.uk.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Sir William Dunn School of Pathology, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27440905</idno><idno type="pmid">27440905</idno><idno type="doi">10.1128/JVI.01384-16</idno><idno type="wicri:Area/PubMed/Corpus">000046</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000046</idno><idno type="wicri:Area/PubMed/Curation">000046</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000046</idno><idno type="wicri:Area/PubMed/Checkpoint">000041</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000041</idno><idno type="wicri:Area/Ncbi/Merge">000C96</idno><idno type="wicri:Area/Ncbi/Curation">000C96</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C96</idno><idno type="wicri:Area/Main/Merge">000400</idno><idno type="wicri:Area/Main/Curation">000400</idno><idno type="wicri:Area/Main/Exploration">000400</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix.</title><author><name sortKey="Long, Joshua C D" sort="Long, Joshua C D" uniqKey="Long J" first="Joshua C D" last="Long">Joshua C D. Long</name><affiliation wicri:level="4"><nlm:affiliation>Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Sir William Dunn School of Pathology, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author><author><name sortKey="Fodor, Ervin" sort="Fodor, Ervin" uniqKey="Fodor E" first="Ervin" last="Fodor">Ervin Fodor</name><affiliation wicri:level="4"><nlm:affiliation>Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom ervin.fodor@path.ox.ac.uk.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Sir William Dunn School of Pathology, University of Oxford, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName><orgName type="university">Université d'Oxford</orgName></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptor Proteins, Signal Transducing (deficiency)</term><term>Adaptor Proteins, Signal Transducing (metabolism)</term><term>Animals</term><term>Fibroblasts</term><term>Influenza A Virus, H1N1 Subtype (enzymology)</term><term>Mice</term><term>Mice, Knockout</term><term>Microscopy, Electron</term><term>Mitochondria (chemistry)</term><term>Protein Sorting Signals</term><term>Protein Transport</term><term>Viral Proteins (analysis)</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Fibroblastes</term><term>Microscopie électronique</term><term>Mitochondries ()</term><term>Protéines adaptatrices de la transduction du signal (déficit)</term><term>Protéines adaptatrices de la transduction du signal (métabolisme)</term><term>Protéines virales (analyse)</term><term>Protéines virales (génétique)</term><term>Signaux de triage des protéines</term><term>Souris</term><term>Souris knockout</term><term>Sous-type H1N1 du virus de la grippe A (enzymologie)</term><term>Transport de protéines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Adaptor Proteins, Signal Transducing</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adaptor Proteins, Signal Transducing</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Protéines adaptatrices de la transduction du signal</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines adaptatrices de la transduction du signal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fibroblasts</term><term>Mice</term><term>Mice, Knockout</term><term>Microscopy, Electron</term><term>Protein Sorting Signals</term><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Fibroblastes</term><term>Microscopie électronique</term><term>Mitochondries</term><term>Signaux de triage des protéines</term><term>Souris</term><term>Souris knockout</term><term>Transport de protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The polymerase basic 2 (PB2) subunit of the RNA polymerase complex of seasonal human influenza A viruses has been shown to localize to the mitochondria. Various roles, including the regulation of apoptosis and innate immune responses to viral infection, have been proposed for mitochondrial PB2. In particular, PB2 has been shown to inhibit interferon expression by associating with the mitochondrial antiviral signaling (MAVS) protein, which acts downstream of RIG-I and MDA-5 in the interferon induction pathway. However, in spite of a growing body of literature on the potential roles of mitochondrial PB2, the exact location of PB2 in mitochondria has not been determined. Here, we used enhanced ascorbate peroxidase (APEX)-tagged PB2 proteins and electron microscopy to study the localization of PB2 in mitochondria. We found that PB2 is imported into mitochondria, where it localizes to the mitochondrial matrix. We also demonstrated that MAVS is not required for the import of PB2 into mitochondria by showing that PB2 associates with mitochondria in MAVS knockout mouse embryo fibroblasts. Instead, we found that amino acid residue 9 in the N-terminal mitochondrial targeting sequence is a determinant of the mitochondrial import of PB2, differentiating the localization of PB2 of human from that of avian influenza A virus strains. We also showed that a virus encoding nonmitochondrial PB2 is attenuated in mouse embryonic fibroblasts (MEFs) compared with an isogenic virus encoding mitochondrial PB2, in a MAVS-independent manner, suggesting a role for PB2 within the mitochondrial matrix. This work extends our understanding of the interplay between influenza virus and mitochondria.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Long, Joshua C D" sort="Long, Joshua C D" uniqKey="Long J" first="Joshua C D" last="Long">Joshua C D. Long</name></region><name sortKey="Fodor, Ervin" sort="Fodor, Ervin" uniqKey="Fodor E" first="Ervin" last="Fodor">Ervin Fodor</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000400 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000400 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27440905
|texte= The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27440905" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |